| 响应面法优化灵芪菌质多糖固态发酵条件 |
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| 引用本文： | 朱强,夏艳秋,陈爱芹,熊晓辉.响应面法优化灵芪菌质多糖固态发酵条件[J].中国生物工程杂志,2011,31(12):99-103. |
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| 作者姓名： | 朱强 夏艳秋 陈爱芹 熊晓辉 |
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| 作者单位： | 1. 淮海工学院 海洋学院 连云港 222005;
2. 南京工业大学 食品与轻工学院 南京 210009 |
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| 摘 要： | 采用单因素试验和响应面分析法优化灵芪菌质多糖固态发酵条件.优化后发酵条件为:采用药性固体发酵培养基,初始pH6.5,接种量10.16ml/100g,发酵温度30.65℃,相对湿度59.54％,发酵周期28d.利用此优化条件进行灵芪菌质多糖发酵,灵芪菌质多糖含量可达9.12±0.09mg/g,较优化前8.06±0.07 ...
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| 关 键 词： | 灵芝 黄芪 灵芪菌质多糖 固态发酵 |
| 收稿时间： | 2011-09-01 |
| 修稿时间： | 2011-10-08 |
Optimization of Ganodetma lucidum-Astragalus membranaceus Polysaccharide Solid Fermentation Condition by Response Surface Analysis |
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| ZHU Qiang,XIA Yan-qiu,CHEN Ai-qin,XIONG Xiao-hui.Optimization of Ganodetma lucidum-Astragalus membranaceus Polysaccharide Solid Fermentation Condition by Response Surface Analysis[J].China Biotechnology,2011,31(12):99-103. |
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| Authors: | ZHU Qiang XIA Yan-qiu CHEN Ai-qin XIONG Xiao-hui |
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| Institution: | ZHU Qiang1 XIA Yan-qiu1 CHEN Ai-qin1 XIONG Xiao-hui2(1 College of Marine,Huaihai Institute of Technology,Lianyungang 222005,China)(2 College of Food Science and Light Industry,Nanjing University of Technology,Nanjing 210009,China) |
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| Abstract: | Single factor test and response surface analysis were used for optimization of Ganodetma lucidum-Astragalus membranaceus polysaccharide solid fermentation. Optimized solid fermentation conditions were as follows: herbs solid fermentation medium, initial pH6.5, inoculum volume 10.16ml/100g, fermentation temperature 30.65℃, relative humidity 59.54%, fermentation period 28d. Under the above optimum conditions, Ganodetma lucidum-Astragalus membranaceus polysaccharide(GAPS) content was up to 9.12 ± 0.09mg/g and increased by 13.15% than unoptimization(8.06±0.07 mg/g), which provided a theoretical experimental basis for pharmacodynamics research and industrialization development. |
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| Keywords: | Ganoderma lucidum Astragalus membranaceus Ganodetma lucidum-Astragalus membranaceus polysaccharide(GAP S) Solid fermentation(SF) |
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