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SPR‐based plastic optical fibre biosensor for the detection of C‐reactive protein in serum
Authors:Ayda Aray  Francesco Chiavaioli  Mojtaba Arjmand  Cosimo Trono  Sara Tombelli  Ambra Giannetti  Nunzio Cennamo  Mahmood Soltanolkotabi  Luigi Zeni  Francesco Baldini
Affiliation:1. Quantum Optics Group, Department of Physics, University of Isfahan, Isfahan, Islamic Republic of Iran;2. Institute of Applied Physics “Nello Carrara”, National Research Council, Sesto Fiorentino, Italy;3. +39 055 522 6359+39 055 522 6400;4. Department of Industrial and Information Engineering, Second University of Naples, Aversa, Italy
Abstract:A plastic optical fibre biosensor based on surface plasmon resonance for the detection of C‐reactive protein (CRP) in serum is proposed. The biosensor was integrated into a home‐made thermo‐stabilized microfluidic system that allows avoiding any thermal and/or mechanical fluctuation and maintaining the best stable conditions during the measurements. A working range of 0.006–70 mg L–1 and a limit of detection of 0.009 mg L–1 were achieved. These results are among the best compared to other SPR‐based biosensors for CRP detection, especially considering that they were achieved in a real and complex medium, i.e. serum. In addition, since the sensor performances satisfy those requested in physiologically‐relevant clinical applications, the whole biosensing platform could well address high sensitive, easy to realize, real‐time, label‐free, portable and low cost diagnosis of CRP for future lab‐on‐a‐chip applications.
figure

3D sketch (left) of the thermo‐stabilized home‐made flow cell developed to house the SPR‐based plastic optical fibre biosensor. Exemplary response curve (shift of the SPR wavelength versus time) of the proposed biosensor (right) for the detection of C‐reactive protein in serum.

Keywords:surface plasmon resonance  plastic optical fibre  C‐reactive protein  serum  microfluidic system
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