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Comparing Raman and fluorescence lifetime spectroscopy from human atherosclerotic lesions using a bimodal probe |
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| Authors: | Sebastian Dochow Hussain Fatakdawala Jennifer E. Phipps Dinglong Ma Thomas Bocklitz Michael Schmitt John W. Bishop Kenneth B. Margulies Laura Marcu Jürgen Popp |
| Affiliation: | 1. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich‐Schiller University Jena, Jena, Germany;2. Department of Biomedical Engineering, University of California, Davis, Davis, USA;3. Department of Pathology & Laboratory Medicine, University of California Davis Medical Center, Davis, CA, USA;4. Cardiovascular Institute, Perelman School of Medicine, , University of Pennsylvania, Philadelphia, PA, USA;5. Leibniz Institute of Photonic Technology Jena e.V., Jena, Germany |
| Abstract: | Fluorescence lifetime imaging (FLIm) and Raman spectroscopy are two promising methods to support morphological intravascular imaging techniques with chemical contrast. Both approaches are complementary and may also be used in combination with OCT/IVUS to add chemical specificity to these morphologic intravascular imaging modalities. In this contribution, both modalities were simultaneously acquired from two human coronary specimens using a bimodal probe. A previously trained SVM model was used to interpret the fluorescence lifetime data; integrated band intensities displayed in RGB false color images were used to interpret the Raman data. Both modalities demonstrate unique strengths and weaknesses and these will be discussed in comparison to histologic analyses from the two coronary arteries imaged.  |
| Keywords: | fluorescence lifetime Raman spectroscopy bimodal imaging fiber probes atherosclerosis |