1. +43 50550 4309+43 50550 4399;2. AIT Austrian Institute of Technology GmbH, Health & Environment Department, Molecular Diagnostics, , Vienna, Austriawww.ait.ac.at;3. Phadia Austria GmbH, Part of Thermo Fisher Scientific Immuno Diagnostics, Vienna, Austria;4. University of Tübingen, Institute of Pharmaceutical Sciences, Tübingen, Germany
Abstract:
We report a new method for detecting human IgG (hIgG) in serum on integrated‐optical Mach‐Zehnder interferometer biosensors realized in a high index contrast polymer material system. In the linear range of the sensor (5–200 nM) we observed excellent signal recoveries (95–110%) in buffer and serum samples, which indicate the absence of matrix effects. Signal enhancement was reached by using secondary anti‐human IgG antibodies, which bind to immobilized target IgGs and allow detecting concentrations down to 100 pM. This polymer based optical sensor is fully compatible with cost‐efficient mass production technologies, which makes it an attractive alternative to inorganic optical sensors.
Graphical abstract of the hIgG measured on polymer based photonic sensors using a direct binding assay and a signal enhancement strategy with secondary antibodies.
