The Induction and Development of Potato Microtubers In Vitroon Media Free of Growth Regulating Substances

Reliable microtuber production has been obtained by culturingnodal explants of potato on Murashige and Skoog medium withoutthe addition of growth regulating substances. The use of sucroseat 8 %, as compared with 4 or 12%, advanced the initiation oftuberization and gave more and larger microtubers. A periodof 1 month under 16 h days, followed by transfer to 8 h photoperiodsgave most rapid microtuber development. A reduction in the totalnitrogen supply or an increase in the ratio of ammonium to nitratereduced the size and number of microtubers Potato, Solanum tubersum L;, microtubers, in vitro induction and development, sucrose, photoperiod, nitrogen nutrition