Rapid and sensitive method for detection of Salmonella strains using a combination of polymerase chain reaction and reverse dot-blot hybridization |
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Authors: | Kazuo Iida Akio Abe Hidenori Matsui Hirofumi Danbara Sachio Wakayama Kazuyoshi Kawahara |
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Institution: | Department of Microbiology and Enzymology, Kluyver Laboratory for Biotechnology, Delft University of Technology, Delft, the Netherlands |
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Abstract: | Abstract Cell-free extracts of Thiobacillus acidophilus catalysed the stoichiometric conversion of tetrathionate to thiosulphate, sulphur and two protons. The pH optimum of the enzyme activity was 3.0 and its temperature optimum 40°C. The enzyme was unstable at 30 and 40°C, at which its activity decreased to zero within 100 and 20 h, respectively. Enzyme activity was not affected by incubation for 1 week on ice or by freezing and thawing of the extract. The K m for tetrathionate was 0.3 mM. Enzyme activity was stimulated by ammonium sulphate up to a concentration of 1M. The results indicate that trithionate hydrolase cannot account for the observed conversion of tetrathionate. |
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Keywords: | Thiobacillus acidophilus Acidophile Sulphur metabolism Polythionate hydrolysis Tetrathionate metabolism Trithionate hydrolase |
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