13N isotope studies of glutamine assimilation pathways in Neurospora crassa. |
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| Authors: | J Caldern A J Cooper A S Gelbard and J Mora |
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| Institution: | Departamento de Ecologia Molecular, Universidad Nacional Autónoma de México, Morelos. |
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| Abstract: | L-amide-13N]glutamine in Neurospora crassa is metabolized to 13N]glutamate by glutamate synthase and to 13N]ammonium by the glutamine transaminase-omega-amidase pathway. The 13N]ammonium released is assimilated by glutamate dehydrogenase and glutamine synthetase, confirming the operation of a glutamine cycle. Most of the nitrogen is retained during cycling between glutamate and glutamine. |
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