SYNCHRONOUS MASS-CULTURE OF CHLORELLA |
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Authors: | MORIMURA, YUJI YANAGI, SUSUMU TAMIYA, HIROSHI |
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Affiliation: | The Tokugawa Institute for Biological Research, Tokyo and The Institute of Applied Microbiology, University of Tokyo |
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Abstract: | A new culture apparatus was constructed to obtain large quantitiesof synchronized cells of Chlorella ellipsoidea. Two flat culturechambers made of lucid acrylate resin (each 20 liters in capacity)were placed in a water thermostat together with a bank of 17daylight fluorescent lamps, and the culture was run by the methodstarting from a homogeneous population of Ds-cells accordingto TAMIYA et al. It was demonstrated that with this apparatusone can obtain as much as 200400 mg (dry weight) eachof algal material at 8 or 9 different stages in one cell cycle.Completely synchronized cell cycles could be repeated as manyas 10 times in one series of experiment, indicating that theapparatus can produce 24g (dry weight) each of algalcells of different developmental stages. (Received April 2, 1964; ) |
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