The life style of Aureobasidium pullulans and the multiple forms of its polygalacturonase |
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| Authors: | Eva Stratilová Mária Dzúrová Emília Breierová Anna Malovíková Ji?ina Omelková |
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| Institution: | (1) Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia;(2) Faculty of Chemistry, Technical University of Brno, Purkyňova 118, CZ-61200 Brno, Czech Republic |
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| Abstract: | The life style of Aureobasidium pullulans on pectin medium and its production of extracellular polygalacturonases are closely related. Polygalacturonases with random
action pattern (EC 3.2.1.15) were formed in the first phases of cultivation, whereas exopolygalacturonases (EC 3.2.1.67) with
terminal action pattern on pectin were produced during the whole growth of this yeast-like fungus. The production and inactivation
of individual enzyme forms during cultivation were strongly dependent on the pH value of the pectin medium. Various kinds
of stress can support the prolongation of the phase of endo-acting enzyme production, as well as the increase of their activity. |
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| Keywords: | Aureobasidium pullulans exopolygalacturonase multiple forms polygalacturonase |
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