Isolation of a heat-labile enterotoxin produced by a human strain of Escherichia coli by wheat-germ agglutinin affinity chromatography |
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| Authors: | F Grasser-Regallet JM Scheftel H Monteil |
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| Institution: | Laboratoire de Toxinologie Bactérienne, Institut de Bactériologie, Facultéde Médecine 3, rue Koeberlé, 67000 Strasbourg, France |
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| Abstract: | Abstract A chromatographic method, wheat-germ agglutinin affinity chromatography, was developed to isolate Escherichia coli heat-labile enterotoxin from human source. Isolated LT enterotoxin showed potent activity in the rabbit jejunal loop assay, and immunological and structural analogies with cholera enterotoxin in the radial immuno-hemolysis test and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. |
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| Keywords: | Escherichia coli heat-labile enterotoxin WGA chromatographic method |
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