In vitro methylation of total and foldback DNAs in normal and virus-transformed cells |
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Authors: | Pietro Voipe Tamilla Eremenko |
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Institution: | Laboratory of Cell and Molecular Biology of the Institute of Experimental Medicine, CNR, Viale R. Elena, 324, RomeItaly;Department of Organic and Biological Chemistry of the University of Naples, Naples, Italy |
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Abstract: | The levels of the in vitro methylation of total and palindromic DNAs in nuclei isolated from normal and virus-transformed cells are compared. The methylation rate of total DNA in normal rat kidney cells is much higher than that detected in normal mouse fibroblasts. However, for both cell species, while the maximal rate of DNA methylation is observed in the mid-logarithmic phase of the cell culture growth, palindromes are always found to be more heavily methylated than total DNA. The 5-methylcytosine content of DNA, especially of palindromes, is higher in virus-transformed cells than in untransformed cells. |
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Keywords: | DNA methylation Palindrome Cell growth cycle Viral transformation |
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