Glycine and asparagme tRNA sequences from the archaebacteriuin,Methanobacterium thermoautotrophicum |
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Authors: | Xiang-Rong Gu K. Nicoghosian R.J. Cedergren |
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Affiliation: | Département de Biochimie, Université de Montréal, Montréal, Québec H3C 3J7, Canada |
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Abstract: | Two tRNA sequences from Methanobacterium thermoautotrophium are reported. Both tRNAGlyGCC and tRNANUUAsn, the first tRNA sequences from methanogens, were determined by partial hydrolyses (both chemical and enzymatic) and analyzed by gel electrophoresis. The two tRNAs contain the unusual T-loop modifications, Cm and m1I, which are present in other archaebacterial tRNAs. Finally the presence of an unknown modification in the D-loop has been inferred by a large jump in the sequence ladder. These tRNAs are approximately equidistant from eubacterial or eukaryotic tRNAs. |
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Keywords: | tRNA sequence Evolution Methanogen |
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