Increase in temperature induces the Z to B transition of poly[d(G-C)] in water-ethanol solution |
| |
Authors: | Armen T Karapetyan Elvira E Minyat Valery I Ivanov |
| |
Institution: | 1. Kirovakan Teachers Training Institute, Kirovakan, Armenian SSR USSR Academy of Sciences, 117984 Moscow B-334, USSR;2. Institute of Molecular Biology, USSR Academy of Sciences, 117984 Moscow B-334, USSR |
| |
Abstract: | An increase in temperature from 20 to 50° C results in the complete transition from the Z to B form of poly(d(G-C)], dissolved in a 55% ethanol-water solution. The transition is fully reversible and displays a slow kinetics. The transition profiles for the free polynucleotide and for that in the presence of ethidium bromide, which is known to stabilize the B form, are obtained by circular dichroism. Based on these data the enthalpy value for the B-Z transition in our conditions is estimated to be ΔHBZ = ?0.7 . |
| |
Keywords: | Poly[d(G-C)] Ethidium bomide Z form Circular dichroism B-Z trantion enthalpy |
本文献已被 ScienceDirect 等数据库收录! |
|