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The effect of phallotoxins on the structure of F-actin in myosin-free ghost muscle fibres of rabbit |
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| Authors: | YuS Borovikov AE Bukatina BJa Son&#x;kir |
| Institution: | 1. Institute of Cytology, Academy of Sciences of the USSR, LeningradUSSR;2. Institute of Biophysics, Academy of Sciences of the USSR. Pustchino-na-Okje, Moscow Region, USSR |
| Abstract: | Using polarized UV fluorescent microscopy it has been shown that phallotoxins (phalloidin-sulfone, phalloidin-sulfoxide-B, phalloidin-sulfoxide-A and dithio-phalloidin) cause an increase in tryptophan fluorescence anisotropy of F-actin myofilaments in myosin-free ghost muscle fibres of rabbit. The results obtained are considered to be evidence of conformational changes in F-actin, induced by phallotoxins. These changes are irreversible to a significant extent, which points to a high degree of actin binding to both toxic and nontoxic phallotoxins. |
| Keywords: | F-Actin conformational change Phallotoxin Ghost fiber Polarized U V microphotometry Ph phalloidin PhS phalloidin-sulfone PhSB phalloidin-sulfoxide-B DTPh dithio-phalloidin PhSA phalloidin-sulfoxide-A SPh seco-phalloidin |
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