| 犬细小病毒中国内蒙株VP2基因克隆及序列分析 |
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| 引用本文： | 董江丽,李淑芬.犬细小病毒中国内蒙株VP2基因克隆及序列分析[J].Virologica Sinica,2000,15(4):379-37. |
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| 作者姓名： | 董江丽 李淑芬 |
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| 作者单位： | [1]内蒙古大学生命科学院,呼和浩特 [2]内蒙古农业大学生物工程系,呼和浩特 |
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| 基金项目： | 内蒙古自然科学基金资助项目!(980 2 0 2 ) |
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| 摘 要： | 从我国内蒙古地区流行的犬细小病毒病病犬的肠溶物中分离提纯犬细小病毒（ＣＰＶ）。提取病毒基因组ＤＮＡ，并以此ＤＮＡ为模板，采用人工合成的引物进行ＰＣＲ扩增，ＰＣＲ产物经ＢａｍＨＩ、ＳａｃＩ双酶切后，克隆于ｐＵＣ１９质粒的ＢａｍＨＩ／ＳａｃＩ位点。重组质粒ｐＵＣＶＰ２经ＰＣＲ鉴定、限制酶切分析和序列分析，结果表明：获得了犬细小病毒内蒙株（ＣＰＶ－ＩＭ）ＶＰ２基因的全长克隆，ＶＰ２基因全长１７５５ｎｔ，
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| 关 键 词： | 犬细小病毒 VP2基因 序列分析 中国内蒙株 |
Molecular Cloning and Sequencing of VP2 Gene of Canine Parvovirus Inner Mongolia Isolate (CPV-IM) |
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| DONG Jiang li ,LI Shu fen ,ZHANG He ling.Molecular Cloning and Sequencing of VP2 Gene of Canine Parvovirus Inner Mongolia Isolate (CPV-IM)[J].中国病毒学(英文版),2000,15(4):379-37. |
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| Authors: | DONG Jiang li LI Shu fen ZHANG He ling |
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| Institution: | DONG Jiang li 1,LI Shu fen 2,ZHANG He ling 1 1 |
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| Abstract: | The DNA of Canine Parvovirus Inner Mongolia isolate (CPV IM) was isolated from the enteric lysate of virus infected dog showing enteritis symptoms. The VP2 gene of CPV IM was amplified by PCR using designed and synthesized two primers and cloned into plasmid pUC19 at Bam H I/ Sac I sites. Recombinant plasmid pUCVP2 was identified by PCR and restriction enzymes analysis and nucleotide sequence analysis. Results show that the full length VP2 gene clone of CPV IM was obtained. VP2 gene of CPV IM isolate consists of 1 755 nt, and there is a high homology in nucleotide sequence and amino acid sequence in comparison with CPV N, CPV B and Feline Panleukopenia Virus (FPLV). The rate of nucleotide sequence homology is 99.32%, 98.29% , 98.52%, and the rate of amino acid homology is 98.97%, 97.09%, 97.77%, respectively. |
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| Keywords: | Canine Parvovirus VP2 gene Nucleotide sequenc |
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