不同CMV分离物侵染寄主的超微结构变化

应用电镜观察了黄瓜花叶病毒ＣＭＶ不同分离物侵染寄主的细胞超微结构变化。来自一患红（Ｓａｌｖｉａｓｐｌｅｎｄｅｎｓ）的不含卫星ＲＮＡ分离物Ｍ－２２侵染心叶烟，病毒粒子散布于细胞质，在液泡中形成大片病毒粒子结果，液泡膜边缘产生小泡结构，完整的病毒粒子穿过胞间连丝在细胞间运转，胞间连丝中央部分有扩张现象。

Ultrastructural Alteration of Host Plants Infected with Different Cucumber Mosaic Virus Isolates

The ultrastructural alterations caused by the infection of two cucumber mosaic virus isolates were investigated. Spherical virus particles of CMV M 22, an isolate originally from Salvia splendens , scattered throughout the cytoplasm and aggregated into crystals in the vacuoles of tobacco cells. The existence of virus particles inside the chloroplast was not detected for this isolate. But virus particles were found being aggregated through the plasmodesma, that indicated the way of cell to cell movement of this isolate. Virus particles of CMV 8 S1, an isolate with necrosis satellite RNA and originally from naturally infected tobacco, scattered throughout the cytoplasm and in the vacuoles of abnormal chloroplasts. In the tobacco leaf cells natural infected by CMV 8 in the field, numerous spherical particles and abnormal chloroplasts were observed. The results suggest that ultrastructural alterations induced by different CMV isolates are quite variable. This is a direct evidence of satellite RNA involvement in pathogenic process by CMV.