| 水稻条纹病毒两个分离物RNA4基因间隔区的序列比较 |
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| 引用本文： | 魏太云,林含新.水稻条纹病毒两个分离物RNA4基因间隔区的序列比较[J].Virologica Sinica,2000,15(2):156-162. |
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| 作者姓名： | 魏太云 林含新 |
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| 作者单位： | [1]福建农业大学植物病毒研究所福建省植物病毒学重点实验室 [2]福建农业大学植物病毒研究所福建省植物病毒学重点实 |
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| 基金项目： | 国家自然科学基金资助项目! (39670 4 89)，福建省自然科学基金资助项目! (C970 31 ) |
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| 摘 要： | 通过ＲＴ－ＰＣＲ扩增了我国水稻秫纹病毒（ＲＳＶ）山东济宁（ＪＮ）、云南宜良（ＹＬ）两个分离物ＲＮＡ４基因间隔区（ｉｎｔｅｒｇｅｎｉｃ ｒｅｇｉｏｎ，ＩＲ）序列，并克隆于ｐＧＥＭ－Ｔ ｅａｓｙ载体上。序列分析结果表明：ＪＮ、ＹＬ两分离物ＲＮＡ４ ＩＲ均由６５４个核苷酸组成，两者之间的同源率为９２％。ＪＮ、ＹＬ两个分离物ＲＮＡ４ ＩＲ在ＡＵ碱基富集处可形成上明显的结构，其中一个序列比较保守，形成的发夹
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| 关 键 词： | 水稻条纹病毒 RNA4基因间隔区 序列 |
Sequence Comparison of Intergenic Region between Two Isolates of Rice Stripe Virus RNA4 |
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| WEI Tai yun,LIN Han xin,WU Zu jian,LIN Qi ying,XIE Lian hui.Sequence Comparison of Intergenic Region between Two Isolates of Rice Stripe Virus RNA4[J].中国病毒学(英文版),2000,15(2):156-162. |
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| Authors: | WEI Tai yun LIN Han xin WU Zu jian LIN Qi ying XIE Lian hui |
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| Abstract: | The RNA4 intergenic regions of two isolates of rice stripe virus (RSV) in China (de signated as RSV JN and RSV YL) were amplified by RT PCR and cloned into pGEM T easy vector. Sequence analysis showed that they were all 654 bp in length, and shared only 92% identity at the nucleotide level. Computer assisted folding analysis showed that the intergenic region was rich in A and U residues where two distant hairpin structures could be formed. One was highly conserved and stable, but the other was rather unstable because of bases variation. Therefore, there were molecular variation among isolates of rice stripe virus in China. |
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| Keywords: | Rice stripe virus RNA4 intergenic regions Sequence comparison |
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