| HCV全长NS3基因表达及在抗体检测中的应用 |
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| 引用本文： | 张斌 叶林柏. HCV全长NS3基因表达及在抗体检测中的应用[J]. Virologica Sinica, 2000, 15(3): 308-311 |
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| 作者姓名： | 张斌 叶林柏 |
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| 作者单位： | 武汉大学病毒学研究所,武汉 |
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| 摘 要： | 丙型肝炎病毒 (ＨＣＶ)是引起非甲非乙型肝炎的主要病原因子。被ＨＣＶ感染的病例中 ,超过 5 0 %以上会引起持续性感染、慢性肝炎 ,最终可能引起肝硬化和肝细胞癌[1] 。ＨＣＶ严重威胁人类健康 ,但目前对丙肝患者尚缺乏有效的治疗手段 ,因此 ,严格把好血源关 ,提高对丙肝患者检出的灵敏度 ,是阻止丙肝血源传播的有效手段。丙型肝炎病毒基因组为单股正链ＲＮＡ ,核苷酸长约 9.5ｋｂ ,仅含一个开放阅读框 ,翻译成一个大的聚蛋白前体 ,由宿主细胞信号肽酶和病毒蛋白酶加工成多个成熟蛋白。其中非结构蛋白ＮＳ3分子量为 70ｋＤ ,有丝氨酸蛋白酶…
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| 关 键 词： | 丙型肝炎病毒 NS3 表达 抗体检测 |
Expression of Full-length HCV NS3 Gene in E.coli and Its Application in HCV Antibody Detection |
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| ZHANG Bin,YE Lin bai,GAO Jin rong,XUE Jing ping,RUAN Hua WANG Xiao ling,ZHAO Yue e. Expression of Full-length HCV NS3 Gene in E.coli and Its Application in HCV Antibody Detection[J]. 中国病毒学(英文版), 2000, 15(3): 308-311 |
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| Authors: | ZHANG Bin YE Lin bai GAO Jin rong XUE Jing ping RUAN Hua WANG Xiao ling ZHAO Yue e |
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| Abstract: | Full length NS3 gene of a hepatitis C virus was amplified by PCR using plasmid pBAC25 containing HCV nonstructural protein gene as template. The amplified fragment (about 1.8 kb) was cloned into plasmid pQE30 and the recombinant plasmid was expressed in JM109. The NS3 protein was purified by NiSO 4 metal chelating resin, and its antigenecity was determined by ELISA, the results showed that the full length NS3 protein was more sensitive than the commercial carboxy terminal domain of NS3 protein in HCV antibody detection. |
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| Keywords: | Hepatitis C virus NS3 Expression Antibody detection |
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