| 戊型肝炎病毒（HEV）开读框架2中1.3kb cDNA的克隆与序列分析 |
| |
| 引用本文： | 蒋琳,杨恭. 戊型肝炎病毒（HEV）开读框架2中1.3kb cDNA的克隆与序列分析[J]. Virologica Sinica, 2000, 15(3): 237-242 |
| |
| 作者姓名： | 蒋琳 杨恭 |
| |
| 作者单位： | [1]卫生部兰州生物制品研究所,兰州 [2]兰州大学生物系,兰州 |
| |
| 摘 要： | 以戊型肝炎（Ｈｅｐａｔｉｔｉｓ）病人高热期血清为原材料，设计特定引物，经ＲＴ－ＰＣＲ扩增获得开放阅读框２（ＯＲＦ２）１．３ｋｂ基因片段，用ＥｃｏＲＩ和ＢａｍＨＩ插入克隆载体ｐＵＣ１８，测定了该克隆基因片段的核苷酸序列并进行了比较分析。其片段长度为１３０９ｂｐ，其Ａ＝２４９（１９．２％），Ｇ＝３１８（２４．２９％），Ｔ＝３３９（２５．９％），Ｃ＝４０３（３０．７９％）；与印度株和缅甸株的同源性在９０
|
| 关 键 词： | 戊型肝炎病毒 cDNA克隆 测序 |
Cloning and Sequence Analysis of the 1.3 kb cDNA Fragment from Open Reading Frame 2 (ORF2) of Hepatitis E Virus (HEV) |
| |
| JIANG Lin ,YANG Gong ,SHEN Xin liang. Cloning and Sequence Analysis of the 1.3 kb cDNA Fragment from Open Reading Frame 2 (ORF2) of Hepatitis E Virus (HEV)[J]. 中国病毒学(英文版), 2000, 15(3): 237-242 |
| |
| Authors: | JIANG Lin YANG Gong SHEN Xin liang |
| |
| Affiliation: | JIANG Lin 1,YANG Gong 2,SHEN Xin liang 1 1 |
| |
| Abstract: | The serum from patients infected with Hepatitis E was prepared, the RNA of Hepatitis E virus was purified. The interested cDNA fragment (1.3?kb) was obtained by RT PCR, cloned in to the plasmid pUC18 and sequenced by the dideoxynucleotide method (fluorescein labeled dNTP). The result of sequencing suggested that the cDNA fragment was 1?309?bp, its adenine, guanine, thymine and cytosine were 249 (19.2%), 318(24.29%), 339(25.9%) and 403(30.79%), respectively. The nucleic acid sequences of the cDNA were 92% to 93.3% homologous to the Indian and Burmese strains, 90.7% and 77.4% homologous to Chad and Mexican strains. The ORF2 of HEV is relatively stable between the Asian strains. |
| |
| Keywords: | Hepatitis E virus cDNA cloning Sequencing |
| 本文献已被 CNKI 维普 等数据库收录！ |
