大熊猫犬瘟热病毒附着或血凝蛋白基因的序列分析

首次对犬瘟热病毒（ＣＤＶ）大熊猫（ＧＰ）毒株附着或血凝蛋白（Ｈ）基因进行了序列测定并与疫苗株Ｏｎｄｅｒｓｔｅｐｏｏｒｔ进行了比较。我们设计合成了４对引物，对ＧＰ株进行了ＲＴ－ＰＣＲ扩增与测序。Ｈ蛋白基因全长为１９４６ｂｐ，开放阅读框架（ＯＲＦ）始于２１－２３位的ＡＴＧ，终止于１８４２－１８４４位的ＴＧＡ，编码６０７个氨基酸，该基因序列已被ＧｅｎＢａｎｋ。将ＧＰ毒株与ＧｅｎＢａｎｋ中疫苗弱毒株Ｏｎｄ

Nucleotide sequecnce Analysis of the Haemagglutinin Protein Gene of Giant Panda Strain of Canine Distemper Virus

The sequence analysis of the haemagglutinin (H) protein gene of giant panda strain of canine distemper virus (CDV) from giant panda in China was conducted and compared with Onderstepoort vaccine strain. Four pairs of primers were designed and synthesized. The PCR products amplified from GP strain were directly sequenced after they were purified. There are 1?946 nt of the complete H gene. The H gene of the GP strain showed that the largest open reading frame (ORF) encoding a protein of 607 amino acid residues started at the ATG at position 21 23 and extended to a termination codon (TGA at position 1?842 1?844). The deduced amino acid sequence of the H protein of the GP strains was compared with the CDV vaccine Onderstepoort strain extracted from the GenBank database. The sequence of the H gene and deduced H protein of the GP strain showed an identity of 91.4% and 90.2% with Onderstepoort strain, respectively. All the 12 cysteine residues found in the GP strain H protein were conserved in Onderstepoort strain. Although predicted hydrophilicity of the GP strain differed from that of the Onderstepoort strain, the predicted transmembrane position at 35 55 amino acid residues was conserved in both strains. Out of the 4 potential N linked glycosylation sites of Onderstepoort H protein, two were located in the N terminal 2/3 of the protein (one located in the putative cytoplasmic part and is probably not used), while the remaining 2 sites were located in the C terminal 1/3 of the protein. Four of the 9 potential N linked glycosylation sites of GP H protein were conserved in the Onderstepoort, but the other five sites are unique to GP, the extra glycosylation sites may influence antigenicity of the GP.