CD157 is part of a supramolecular complex with CD11b/CD18 on the human neutrophil cell surface |
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Authors: | Lavagno L Ferrero E Ortolan E Malavasi F Funaro A |
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Affiliation: | Laboratory of Immunogenetics, Department of Genetics, Biology and Biochemistry, University of Torino, Torino, Italy. |
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Abstract: | CD157 is a GPI-anchored cell surface glycoprotein expressed by human peripheral blood neutrophils. Cross-linking of CD157 induces intracellular Ca2+ mobilization and re-shaping in neutrophils, thus regulating their adhesive and migratory properties. Results obtained by immunolocalization and confocal microscopy indicate that CD157 lies in close proximity to the CD11b/CD18 complex which is strongly expressed on the activated neutrophil cell membrane where it plays a predominant role in adhesion. This study analyses the physical association between CD157 and CD18 in human neutrophils by co-immunoprecipitation experiments. The anti-CD157 monoclonal antibody RF3 co-precipitates CD18, and the anti-CD18 antibody TS1/18 co-precipitates CD157 from human neutrophil lysates. These results confirm that CD157 physically interacts with CD11b/CD18 complex in human neutrophils. |
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