Identification of leucocyte surface antigens in paraffin- embedded bovine tissues using a modified formalin dichromate fixation |
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Authors: | Birgit Rathkolb Joachim F Pohlenz Peter Wohlsein |
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Institution: | (1) Institut fur Pathologie, Tierarztliche Hochschule Hannover, Bunteweg 17, D-30559 Hannover, Germany |
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Abstract: | A modified fixative of formalin dichromate was combined with a cold embedding procedure for the preservation of bovine leucocyte
surface antigens. Fourteen monoclonal antibodies recognizing seven bovine leucocyte surface antigens (BoCD1w2, BoCD4, BoCD8,
BoWC1, BoWC3, BoWC4 and BoIgM) were applied as primary antisera in a sensitive avidin--biotin--peroxidase complex detection
method. The staining results were compared with those obtained in cryostat and routinely formalin-fixed sections of corresponding
tissue samples. Using the modified formalin dichromate fixative and the cold embedding procedure, all the leucocyte surface
antigens tested were detectable immunohistologically in paraffin sections with a generally more distinct staining than in
traditionally processed tissues. Morphological structures were better preserved than in cryostat sections but, to some extent,
were poorer when compared with routinely formalin-fixed tissues. However, this method suggests that there are only mild masking
effects and provides an alternative to the use of unfixed material, particularly for morphological--immunohistochemical investigations |
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