Chemical composition of Desulfovibrio desulfuricans lipid A |
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Authors: | Daniel Wolny Jolanta Lodowska Marzena Jaworska-Kik Sławomir Kurkiewicz Ludmiła Węglarz Zofia Dzierżewicz |
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Affiliation: | (1) Department of Biopharmacy, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 St., 41-200 Sosnowiec, Poland;(2) Department of Biochemistry, Faculty of Pharmacy, Medical University of Silesia, Katowice, Poland;(3) Department of Instrumental Analysis, Faculty of Pharmacy, Medical University of Silesia, Katowice, Poland |
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Abstract: | Lipopolysaccharides also called endotoxins are an integral component of the outer membrane of Gram-negative bacteria. When released from the bacterial surface, they interact with a host immune system, triggering excessive inflammatory response. Lipid A is the biologically most active part of endotoxin, and its activity is modulated by the quantity, quality and arrangement of its fatty acids. Desulfovibrio desulfuricans is sulfate-reducing, Gram-negative bacterium that is supposed to be opportunistic pathogens of humans and animals. In the present study, chemical composition of lipid A from various strains of D. desulfuricans was analyzed by gas chromatography/mass spectrometry. It was found that the fatty acid component of the lipid A contains dodecanoic, tetradecanoic, 3-hydroxytetradecanoic and hexadecanoic acids, and its carbohydrate core is composed of glucosamine. The analysis of 3-acyloxyacyl residue of the lipid A revealed the presence of amide-bound 3-(dodecanoyloxy)tetradecanoic and 3-(hexadecanoyloxy)tetradecanoic acids and ester-bound 3-(tetradecanoyloxy)tetradecanoic acid. It was concluded that both fatty acid and 3-acyloxyacyl residue profiles of the lipid A from the studied bacteria were similar to those of E. coli and S.enterica. |
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