Conformational changes induced by Mg2+ on actin monomers. An immunologic attempt to localize the affected region |
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| Authors: | C Roustan Y Benyamin M Boyer R Bertrand E Audemard J Jauregui-Adell |
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| Affiliation: | Centre de Recherches de Biochimie Macromoléculaire, CNRS, Montpellier, et Laboratoire de Biochimie et Ecologie des Invertébrés Marins de l''Ecole Pratique des Hautes Etudes, BP 5051, 34033 Montpellier Cédex, France |
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| Abstract: | The effect of Mg2+ ions on the conformation of G-actin and in particular on the accessibility of its antigenic regions has been tested. Experiments were performed with G-actin coupled to Sepharose 4B which was, therefore, maintained in the monomeric state. The results presented her show that the 2mM MgCl2-perturbed antigenic site is located in a central region of the actin sequence. |
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