Sedimentation equilibrium of DNA samples heterogeneous in density

The problem of determining the molecular weight of DNA samples by sedimentation equilibrium in a buoyant-density gradient is considered for the case of DNA samples with density heterogeneity. By determining apparent molecular weights in two or more buoyant mediums, quantitative measure of the amount of density heterogeneity can be determined. This method may be employed to determine both the true molecular weight and the extent of base composition heterogeneity.