Cyclic Nucleotide-Dependent Protein Kinases and Some Major Substrates in the Rat Cerebellum After Neonatal X-Irradiation |
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Authors: | Annette C Dolphin John A Detre Doris J Schlichter Angus C Nairn Hermes H Yeh† Donald J Woodward† Paul Greengard |
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Institution: | Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut;Department of Cell Biology, The University of Texas Health Science Center at Dallas, Dallas, Texas, U.S.A. |
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Abstract: | The levels of cAMP-dependent protein kinase (type I), or cGMP-dependent protein kinase, or protein I, and of a 23,000 MW substrate for the cGMP-dependent protein kinase were measured in cerebella from normal rats and in the cerebella from rats in which a selective loss of interneurons in the cerebellar cortex had been produced by X-irradiation. A decrease was observed in the concentrations of cAMP-dependent protein kinase and of protein I, whereas an increase was observed in the concentrations of cGMP-dependent protein kinase and of the 23,000 MW substrate. The data, taken together with the results of other studies, support the interpretation that cAMP-dependent protein kinase and protein I are distributed throughout the cerebellum, but that cGMP-dependent protein kinase and the 23,000 MW substrate are highly concentrated in Purkinje cells. |
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Keywords: | cAMP cGMP Phosphorylation Cerebellum-X-irradiation |
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