| 地衣芽孢杆菌D-1产碱性蛋白酶培养条件的优化 |
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| 引用本文： | 刘海进,钱坤,李吕木,许发芝,丁小玲,景志远.地衣芽孢杆菌D-1产碱性蛋白酶培养条件的优化[J].激光生物学报,2011,20(3):409-412,387. |
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| 作者姓名： | 刘海进 钱坤 李吕木 许发芝 丁小玲 景志远 |
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| 作者单位： | 1. 安徽农业大学动物科技学院,安徽合肥230036;安徽省畜牧生物工程技术研究中心,安徽合肥230031
2. 安徽省畜牧生物工程技术研究中心,安徽合肥,230031 |
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| 基金项目： | 国家科技部科技人员服务企业行动项目，安徽省农业科技成果转化项目 |
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| 摘 要： | 为了对产碱性蛋白酶的地衣芽孢杆菌D-1的培养条件进行优化,利用10 L发酵罐,采用正交设计19(34)试验,对培养温度、pH值、搅拌转速、通气量4条件进行优化,得到地衣芽孢杆菌D-1发酵产碱性蛋白酶的最优培养条件为:培养温度37.0℃,pH值7.5,通气量4L/min,搅拌转速300r/min.利用最优条件组合进行验证...
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| 关 键 词： | 地衣芽孢杆菌 碱性蛋白酶 优化 |
Optimization of Culture Conditions of Alkaline Protease Production by Bacillus licheniformis D-1 |
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| LIU Hai-jin,QIAN Kun,LI Lü-mu,XU Fa-zhi,DING Xiao-ling,JING Zhi-yuan.Optimization of Culture Conditions of Alkaline Protease Production by Bacillus licheniformis D-1[J].ACTA Laser Biology Sinica,2011,20(3):409-412,387. |
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| Authors: | LIU Hai-jin QIAN Kun LI Lü-mu XU Fa-zhi DING Xiao-ling JING Zhi-yuan |
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| Institution: | LIU Hai-jin~(1,2),QIAN Kun~2,LI L(u|¨)-mu~(1,2*),XU Fa-zhi~(1,DING Xiao-ling~(1,JING Zhi-yuan ~(1,2) (1.School of Animal Science and Technology,Anhui Agriculture University,Hefei 230036,Anhui,China,2.Anhui Animal Bioengineering Technology Research Center,Hefei 230031,China) |
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| Abstract: | In order to optimize culture condition for alkaline protease production by Bacillus licheniformis D-1, the culture temperature, pH, stirring speed and air flow rate during fermentation were optimized by the L9 （34） orthogonal test in 10 L bioreactor. The optimized culture conditions were determined as a culture temperature of 37 ℃ , a pH of 7, 5, an air flow rate 4 L/min, and a stirring speed of 300 r/min. Under these optimization conditions, the confirmative experiment shows that the activity of alkaline protease produced by Bacillus licheniformis D-1 reached 91.26 U/mL. |
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| Keywords: | Bacillus licheniformis alkaline protease optimization |
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