| 砀山酥梨石细胞发育过程中PPO酶学特性及其cDNA片段克隆 |
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| 引用本文： | 李玲,于娟娟,金青,蔡永萍,林毅,吕芮宏. 砀山酥梨石细胞发育过程中PPO酶学特性及其cDNA片段克隆[J]. 激光生物学报, 2011, 20(3): 353-359. DOI: 10.3969/j.issn.1007-7146.2011.03.015 |
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| 作者姓名： | 李玲 于娟娟 金青 蔡永萍 林毅 吕芮宏 |
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| 作者单位： | 安徽农业大学生命科学学院,安徽合肥,230036 |
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| 摘 要： | 以砀山酥梨果实为材料,研究了影响石细胞形成的木质素合成酶-多酚氧化酶(polyphenol oxidase,PPO)的酶学特性,并对PPO基因进行了克隆.结果表明,在砀山酥梨果实发育的过程中,PPO活性在花后27 d和47 d出现高峰,早于木质素含量高峰(花后47 d、61 d)和石细胞团含量高峰(花后51 d),且P...
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| 关 键 词： | 砀山酥梨 多酚氧化酶 木质素 石细胞 酶学特性 RT-PCR |
PPO Characterization and a PPO cDNA Coloning during the Development of Stone Cell in Pyrus bretschneideri cv. |
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| LI Ling,YU Juan-juan,JIN Qing,CAI Yong-ping,LIN Yi,LV Rui-hong. PPO Characterization and a PPO cDNA Coloning during the Development of Stone Cell in Pyrus bretschneideri cv.[J]. ACTA Laser Biology Sinica, 2011, 20(3): 353-359. DOI: 10.3969/j.issn.1007-7146.2011.03.015 |
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| Authors: | LI Ling YU Juan-juan JIN Qing CAI Yong-ping LIN Yi LV Rui-hong |
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| Affiliation: | LI Ling,YU Juan-juan,JIN Qing,CAI Yong-ping,LIN Yi~*,LV Rui-hong (School of Life Science,Anhui Agricultural University,Hefei 230036,Anhui,China) |
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| Abstract: | The enzymatic characteristics of Polyphenol oxidase(PPO) affected the formation of stone cell were investigated and the PPO gene was cloned with the material of fruit of Pyrus bretschneideri cv.The results showed that during the development of Pyrus bretschneideri cv.,PPO activity reached peaks in 27th and 47th days after anthesis(DAA) respectively, which appeared earlier than the peaks of contents of lignin(in 47 DAA and 61 DAA) and stone cell(in 51 DAA) respectively,meanwhile,the variation of PPO activite... |
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| Keywords: | RT-PCR |
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