| 620nm低能量红光对骨髓间充质干细胞成骨分化的影响 |
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| 引用本文： | 彭飞,郑亚东,徐西强,虞冀哲,吴华. 620nm低能量红光对骨髓间充质干细胞成骨分化的影响[J]. 激光生物学报, 2011, 20(3): 285-288. DOI: 10.3969/j.issn.1007-7146.2011.03.001 |
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| 作者姓名： | 彭飞 郑亚东 徐西强 虞冀哲 吴华 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院矫形外科,湖北武汉,430030 |
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| 摘 要： | 为研究发光二极管(LED)发射的红光对大鼠骨髓间充质干细胞的成骨分化的影响,体外培养SD大鼠骨髓间充质干细胞,620 nm波长的LED置于细胞上方2 cm处,照射剂量分别为0,1,2和4J/cm2.采用CCK-8法检测照射后第2、4天的增殖活性,碱性磷酸酶(ALP)活性试剂盒与von kossa矿化结节染色法检测骨髓间...
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| 关 键 词： | 骨髓间充质干细胞 增殖 分化 光生物调节作用 |
620 nm Red Light Enhances Osteogenic Differentiation in Mesenchymal Stem Cells |
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| PENG Fei,ZHENG Ya-dong,XU Xi-qiang,YU Ji-zhe,WU Hua. 620 nm Red Light Enhances Osteogenic Differentiation in Mesenchymal Stem Cells[J]. ACTA Laser Biology Sinica, 2011, 20(3): 285-288. DOI: 10.3969/j.issn.1007-7146.2011.03.001 |
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| Authors: | PENG Fei ZHENG Ya-dong XU Xi-qiang YU Ji-zhe WU Hua |
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| Affiliation: | PENG Fei,ZHENG Ya-dong,XU Xi-qiang,YU Ji-zhe,WU Hua~* (Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Huhei,China) |
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| Abstract: | To investigate the effect of red light emitted from light-emitting diode(LED) on bone marrow Mesenchymal stem cells(MSCs) cultured with osteogenic supplements,MSCs were divided into four groups and each group was irradiated at dose of 0,1,2,and 4 J/cm~2.Cellular proliferation was evaluated by using CCK-8.The alkaline phosphatase (ALP) activity and mineralization formation were monitored as indicators of MSCs' differentiation toward osteoblasts.As a result,the cellular proliferation was decelerated in 4 J/cm... |
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| Keywords: | mesenchymal stem cells proliferation differentiation photobiomodulation |
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