| 肿瘤转移抑制蛋白MTss1shRNA慢病毒载体的构建与鉴定 |
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| 引用本文： | 毛亚江,蒋鹏,梁宋平,王迎. 肿瘤转移抑制蛋白MTss1shRNA慢病毒载体的构建与鉴定[J]. 激光生物学报, 2011, 20(3): 373-377. DOI: 10.3969/j.issn.1007-7146.2011.03.018 |
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| 作者姓名： | 毛亚江 蒋鹏 梁宋平 王迎 |
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| 作者单位： | 湖南师范大学生命科学学院,湖南长沙,410081 |
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| 基金项目： | 教育部科学技术研究重点项目，湖南省自然科学基金资助项目，湖南师范大学青年优秀人才培养计划 |
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| 摘 要： | 肿瘤转移抑制蛋白MTss1在小脑浦肯野神经元发育中表达受到调控,然而功能并不清楚.拟通过RNAi的方法降低内源MTss1的表达,为MTss1在神经元中的功能研究建立基础.针对小鼠MTss1基因(Mtss1)的mRNA序列,设计合成三对shRNA序列,克隆到pSuper-Retro-Puro质粒中,瞬间转染GFP-Mts...
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| 关 键 词： | MTss1 shRNA 慢病毒 |
Construction and Identification of Lentiviral RNA Interference Vector of Mouse MTss1 Gene |
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| MAO Ya-jiang,JIANG Peng,LIANG Song-ping,WANG Ying. Construction and Identification of Lentiviral RNA Interference Vector of Mouse MTss1 Gene[J]. ACTA Laser Biology Sinica, 2011, 20(3): 373-377. DOI: 10.3969/j.issn.1007-7146.2011.03.018 |
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| Authors: | MAO Ya-jiang JIANG Peng LIANG Song-ping WANG Ying |
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| Affiliation: | MAO Ya-jiang,JIANG Peng,LIANG Song-ping,WANG Ying~* (College of Life Science,Hunan Normal University,Changsha 410081,Hunan,China) |
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| Abstract: | Metastasis suppressor 1 MTss1,as a developmentally regulated gene,has been implicated in the neurogenesis of cerebellar purkinje neuron but the function remains unclear.The aim in this study is to knock down the expression of endogenous MTss1 through RNA interference strategy.Three pair of target sequence were designed and synthesized, then cloned into pSuper-Retro-Puro vector.The knock down efficiencies were further checked by transient transfection and western blot.The shRNA containing fragments from pSup... |
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| Keywords: | MTss1 shRNA |
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