| 卵泡液蛋白质组学2-DE体系的构建与应用 |
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| 引用本文： | 戴国,卢光琇.卵泡液蛋白质组学2-DE体系的构建与应用[J].激光生物学报,2011,20(3):404-408,359. |
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| 作者姓名： | 戴国 卢光琇 |
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| 作者单位： | 1. 中南大学生殖与干细胞工程研究所,湖南长沙410078;湖南师范大学生命科学学院,湖南长沙410081
2. 中南大学生殖与干细胞工程研究所,湖南长沙,410078 |
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| 基金项目： | 国家自然科学基金项目，科技厅院士基金 |
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| 摘 要： | 卵泡液是在卵泡形成过程中生成的,作为卵母细胞生长和分化的培养基,直接或间接影响卵母细胞的生育力和发育潜能,其成分非常复杂。本研究通过调整,优化2-DE技术条件,建立了相对稳定的卵泡液蛋白质2-DE技术,获得了卵泡液蛋白质组表达图谱,并用PDQuest软件进行了图像分析,用MALDI-TOF MS鉴定了9个蛋白点,其中有四种为新鉴定的在卵泡液中表达的蛋白质。结果表明,该体系稳定性、重复性好,为进一步开展卵泡液蛋白质组学的相关研究奠定了基础。
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| 关 键 词： | 双向凝胶电泳 卵泡液 蛋白质组学 白蛋白 IgG |
Establishment of Two-dimensional Polyacrylamide Gel Electrophoresis for Follicular Fluid Proteomics |
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| DAI Guo,LU Guang-xiu.Establishment of Two-dimensional Polyacrylamide Gel Electrophoresis for Follicular Fluid Proteomics[J].ACTA Laser Biology Sinica,2011,20(3):404-408,359. |
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| Authors: | DAI Guo LU Guang-xiu |
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| Institution: | DAI Guo ~(1,2),LU Guang-xiu~(1*) (1.Institute of Reproductive & Stem Cell Engineering,Central South University,Changsha 410078,Hunan,China,2.College of Life Science,Hunan Normal University,Changsha 410081,China) |
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| Abstract: | Human follicular fluid(hFF),which is generated in the process of follicle formation,contains a vast variety of substances.As a medium of oocyte growth and differentiation,it directly or indirectly affects the fertility and developmental potential of oocytes.Through adjusting and optimizing 2-DE technical conditions,this study has established a relatively stable hFF protein 2-DE technology with stability and reproducibility,9 protein spots in a silver-stained gel of 2-DE of human follicular fluid were analyz... |
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| Keywords: | IgG |
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