Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-sigma 37, E-sigma 32) |
| |
Authors: | Christine L. Truitt Elizabeth A. Weaver William G. Haldenwang |
| |
Affiliation: | (1) Department of Microbiology, University of Texas Health Science Center, 78284 San Antonio, TX, USA |
| |
Abstract: | Summary The E-37 gene ctc was inactivated by a site-specific insertion into the Bacillus subtilis chromosome. The resulting mutation inhibited sporulation by 95% at elevated temperatures (48° C). If the ctc- mutation is placed in a strain that carries a mutation in the closely linked but distinct spoVC gene, ctc now affects both growth and sporulation at elevated temperatures. Growth of the ctc- spoVC285 strain was transiently inhibited when exponentially growing cultures were shifted from 37° C to 48° C. A similar, but less pronounced growth lag, was also seen in a B. subtilis strain carrying only the spoVC-285 mutation. This finding suggests that both the ctc and spoVC products function in vegetatively growing B. subtilis. |
| |
Keywords: | Bacillus Sporulation RNA polymerase |
本文献已被 SpringerLink 等数据库收录! |