Detection of carriers for duchenne muscular dystrophy. Quality control of creatine kinase assay |
| |
Authors: | H. Plauchu C. Junien I. Maire R. Said R. Gozlan J. M. Lalouel |
| |
Affiliation: | (1) Chaire de Génétique Médicale (Prof. J. M. Robert), Hotel Dieu, 69288 Lyon cedex 1, France;(2) Institut de Pathologic Moléculaire (Prof. G. Schapira), INSERM U.15, 24 rue du Faubourg Saint Jacques, 75654 Paris cedex 14, France;(3) Laboratoire d'Enzymologie (Prof. J. Cotte), Hopital Debrousse, 69005 Lyon, France;(4) Laboratoire d'Anthropobiologie, Université Paris 7, Tour 16, 2, place Jussieu, 75251 Paris cedex 5, France |
| |
Abstract: | Summary A report by Bullock and coworkers has emphasized the need for standardization of the CK assay in carrier detection for DMD. A collaborative study, according to a well-specified design and involving two laboratories in Paris and Lyon, indicates that the reliability of this assay can be improved provided that special attention is paid to information about participating subjects, laboratory protocol, and repeated sampling. On a random sample of young women, mean and variance of 1n(CK) are 1.66 and 0.015 respectively. Within and between variance components are in a 3:4 ratio, homogeneous between populations. Additional use of a common test serum should insure good reliability of this assay among laboratories. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|