Domain conservation in several volvocalean cell wall proteins |
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Authors: | Jeffrey P. Woessner Arthur J. Molendijk Piet van Egmond Frans M. Klis Ursula W. Goodenough Michel A. Haring |
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Affiliation: | (1) Department of Biology, Washington University, Box 1229, 63130 St. Louis, MO, USA;(2) Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, Netherlands |
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Abstract: | Based on our previous work demonstrating that (SerPro)x epitopes are common to extensin-like cell wall proteins in Chlamydomonas reinhardtii, we looked for similar proteins in the distantly related species C. eugametos. Using a polyclonal antiserum against a (SerPro)10 oligopeptide, we found distinct sets of stage-specific polypeptides immunoprecipitated from in vitro translations of C. eugametos RNA. Screening of a C. eugametos cDNA expression library with the antiserum led to the isolation of a cDNA (WP6) encoding a (SerPro)x-rich multidomain wall protein. Analysis of a similarly selected cDNA (VSP-3) from a C. reinhardtii cDNA expression library revealed that it also coded for a (SerPro)x-rich multidomain wall protein. The C-terminal rod domains of VSP-3 and WP6 are highly homologous, while the N-terminal domains are dissimilar; however, the N-terminal domain of VSP-3 is homologous to the globular domain of a cell wall protein from Volvox carteri. Exon shuffling might be responsible for this example of domain conservation over 350 million years of volvocalean cell wall protein evolution. |
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Keywords: | cell wall HRGPs domain conservation exon shuffling protein evolution Chlamydomonas |
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