The effects of cold-pretreatment,auxins and carbon source on anther culture of rice |
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| Authors: | Trejo-Tapia Gabriela Maldonado Amaya Uriel Salcedo Morales Guadalupe De Jesús Sánchez Antonia Martínez Bonfil Blanca Rodríguez-Monroy Mario Jiménez-Aparicio Antonio |
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| Institution: | (1) Centro de Desarrollo de Productos Bioticos, Instituto Politecnico Nacional, P. O. Box 24, Yautepec, Morelos, Mexico Z.C, 62731;(2) Campo Experimental Zacatepec, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Km. 0.5 Carretera Zacatepec-Galeana, Zacatepec, Morelos, Mexico, Z.C, 62780 |
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| Abstract: | The effects of a cold pretreatment, the concentration of different auxins (2,4-D, NAA and IAA) and the type of carbon source (maltose and sucrose) on the induction of callus from anthers of three parental lines and four rice F1 hybrids (Japonica × Indica, Indica × Japonica) were studied. The results indicated that a cold pretreatment was essential for the induction of callus from anthers of the parental lines and the F1 hybrids. These effects were genotype dependent. Auxins were essential for the induction of callus, and the type and concentration of auxins also influenced this process, as well as the type of carbon source. The greatest induction of callus was by the hybrid Morelos A92 × Koshihikari after a cold pretreatment of 8 days using 10.74  M  –napthaleneacetic acid and 30 g l–1 maltose. |
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| Keywords: | callus induction 2 4-D haploids IAA maltose NAA Oryza sativa L plant growth regulators sucrose |
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