Induction of myosin light chain synthesis in heterokaryons between normal diploid cells

Summary Quail myoblasts were maintained in an undifferentiated state by first blocking differentiation with 5-bromodeoxyuridine and then reversing the block in the presence of phorbol-12-myristate-13-acetate. The synthesis of quail skeletal myosin light chain 1 is induced in heterokaryons formed by fusing these undifferentiated quail myoblasts to differentiated chick myocytes. These results extend observations previously obtained using an established line of rat myoblasts and indicate that the induction is a result of regulatory interactions present in normal diploid cells. This work was supported by grants from the Muscular Dystrophy Association and the National Institutes of Health.