Mucin granules are in close contact with tubular elements of the endoplasmic reticulum. |
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| Authors: | Juan Perez-Vilar Carla M Pedrosa Ribeiro Wendy C Salmon Raean Mabolo Richard C Boucher |
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| Institution: | Cystic Fibrosis/Pulmonary Research and Treatment Center, School of Medicine, University of North Carolina at Chapel Hill, 27599-7248, USA. juan_vilar@med.unc.edu |
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| Abstract: | Live cell imaging methods were used to characterize goblet cells expressing a MUC5AC domain fused to enhanced green fluorescent protein that labels the granule lumen. Golgi complex and endosome/lysosome elements largely resided in the periphery of the granular mass. On the contrary, a tubular meshwork of endoplasmic reticulum (ER) was in close contact with the mucin granules. This meshwork could be identified in fixed native human bronchial goblet cells labeled with an anti-calreticulin antibody. The potential biological significance of this ER network for mucin secretion is discussed. |
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