红花玉兰与玉兰亚属几个种亲缘关系的AFLP分析

应用AFLP分子标记技术对红花玉兰与玉兰亚属几个种之间的亲缘关系，以及红花玉兰的分类地位进行了分析。9对引物对7个玉兰种的36个代表样株进行选择性扩增，共得到扩增谱带874条，其中多态性谱带635条。分析结果表明：各玉兰种间，红花玉兰与白玉兰、武当木兰的遗传相似度较高；玉兰亚属种间基于AFLP分析的聚类结果与形态学对种的划分基本吻合。红花玉兰不仅形态上与其它玉兰种有明显区别，AFLP分子标记也支持红花玉兰为一个独立的新种，多瓣红花玉兰变种与红花玉兰原变种为一个种。

Phyiogenetic Relationship Between Magnolia wufengensis and Several Related Species in Subgenus Yulania Based on AFLP Marker Analysis

The relationship of Magnolia wufengensis with several other magnolia species in subgenus Yulania, and the taxon status of M. wufengensis were analyzed with AFLP molecular marker. A collection of 36 accessions representative of 7 magnolia species were selectively amplified and the results showed that 635 out of 874 bands are polymorphic with nine primer combinations; there was a higher genetic similarity between M. wufengensis and M. denudata, M. sprengeri than other magnolia species. The cluster result based on AFLP was generally in harmony with the morphologic taxa in Subgenus Yulania. Not only distinct morphologic differences from other magnolia species but also AFLP molecular markers supported that M. wufengensis should be a new species and M. wufengensis var. wufengensis and M. wufengensis var. multitepala should be the same one species.