Genetic identification of three species of the genus Clarias using allozyme and mitochondrial DNA markers |
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| Authors: | V Mohindra R K Singh M Palanichamy A G Ponniah K K Lal |
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| Institution: | National Bureau of Fish Genetic Resources (ICAR), Lucknow (UP), India |
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| Abstract: | Genetic variability in three clariid species, Clarias batrachus, C. gariepinus and C. macrocephalus, were investigated by allozyme electrophoresis and mitochondrial DNA (ND 5/6) RFLP markers. The catfish species C. batrachus and C. macrocephalus are native to the Indian subcontinent and Southeast Asia, respectively, while the African catfish C. gariepinus was introduced to Asian countries for culture. Sixteen gene loci from 12 enzyme systems were analyzed. Fixed allelic differences were evident between pairs of species at least at four loci. In mtDNA RFLP analysis, eight composite haplotypes were observed and each species was characterized by a set of haplotypes. The UPGMA phylogenetic tree revealed three distinct clusters: C. batrachus; C. gariepinus (India and Thailand); and C. macrocephalus. |
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