Purification and characterization of human urine-derived digitalis-like factor |
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| Authors: | A Goto K Yamada M Ishii M Yoshioka T Ishiguro C Eguchi T Sugimoto |
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| Affiliation: | Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan. |
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| Abstract: | We were able to purify a digitalis-like factor to apparent homogeneity from human urine based on the inhibitory effect on [3H]-ouabain binding to intact human erythrocytes. This ouabain displacing compound closely resembles ouabain in its polarity, molecular weight, non-peptidic nature and mode of action except for its UV absorbance spectrum. This compound sharing many biological activities of ouabain may be the endogenous ligand for the Na+, K+-ATPase and serve as a specific regulator of the sodium pump. |
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