北京东灵山地区不同海拔柴胡居群的遗传多样性

利用ISSR技术对北京东灵山地区不同海拔的6个柴胡居群进行分析，观察不同海拔柴胡居群的遗传多样性。结果表明，东灵山地区不同海拔柴胡居群的遗传多样性差异较大，海拔1135m居群表现出较高的遗传变异水平，高海拔居群遗传多样性较低；遗传距离与海拔差距有一定的相关性，相邻海拔的不同柴胡居群间具有相对较低的遗传距离；柴胡具有较强的遗传分化趋势，且大部分变异存在于居群内。不同海拔导致的异质生境及基因流是影响柴胡居群遗传多样性的主要原因。

Genetic Diversity of Bupleurum chinense DC.Populations from Different Altitudes in Dongling Mountain District in Beijing

Genetic diversity of Bupleurum chinense DC.populations from different altitudes in Dongling Mountain district in Beijing were conducted by ISSR technology.The values of genetic diversity indexes,Shannon diversity indexes,hereditary distance,genetic differentiation coefficient and gene flow were observed.Results showed that altitude affected the genetic diversity of Bupleurum chinense DC.in Dongling Mountain district.The population at altitude 1135m showed higher genetic variation,while lower genetic diversity was observed in populations of high altitude.Hereditary distances between different populations were certainly correlated with altitude distance,that the value of hereditary distances between near altitude populations was lower.B.chinense DC.has large genetic differentiation,while a majority of genetic variation was existed in inner population.Divergent habitats and gene flow caused by different altitudes played important roles in genetic diversity of B.chinense DC.population.