Oil/mineral-salts medium designed for easy recovery of extracellular lipase from Fusarium oxysporum AM3 |
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| Authors: | Camargo-de-Morais MM Maia MMD Borba FFS Melo KG Santos CMSO Reis ERA Morais MA Lima-Filho JL |
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| Institution: | (1) Setor de Biotecnologia/LIKA, Pernambuco, Brasil; Instituto de Ciências Biológicas, Universidade de Pernambuco, Brasil; Fax: |
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| Abstract: | Lipase production by the potato pathogen Fusarium oxysporum AM3 was investigated in a mineral medium using triolein and sodium nitrate as carbon and nitrogen sources, respectively. Medium design by factorial analysis of the medium components increased enzyme activity 9.4-folds over the standard medium. The simple medium composition promoted easy enzyme recovery to its homogeneity in a single step. The lipase showed optimum activity at pH 9.0 and 35 °C, with a K
M value of 7.5 mM for triolein and apparent molecular weight of 29.0 kDa. When assayed with different solvents, FoxAM3 lipase showed an increase on its activity by isooctane, isopropanol and acetone. |
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| Keywords: | Enzyme production factorial analysis Fusarium lipase protein purification |
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