Two separate tyrosine protein kinases in human platelets |
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| Authors: | S Nakamura F Takeuchi T Tomizawa N Takasaki H Kondo H Yamamura |
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| Affiliation: | 1. Department of Biochemistry, Fukui Medical School, Matsuoka, Fukui 910-11 Nagasaki 825, Japan;2. Department of Industrial Chemistry, Faculty ofEngineering, Nagasaki University, Bunkyo, Nagasaki 825, Japan |
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| Abstract: | Tyrosine protein kinase activities were detected in the cytosolic fraction (PC-TPK) and the particulate fraction (PM-TPK) in human platelets using the synthetic peptide, E11G1 (Glu-Asp-Ala-Glu-Tyr-Ala-Ala-Arg-Arg-Arg-Gly) as a substrate. PC-TPK and PM-TPK were different in substrate specificities, divalent cation requirements and apparent Mr values. These results strongly suggest that in platelets there exist at least two separate tyrosine protein kinases; one is present in cytosol and the other might be associated with membranes. |
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