埃博霉素B小试发酵罐发酵条件研究

研究了纤维堆囊菌(Sorangium cellulosum)So F5-76在5 L发酵罐水平上发酵生产埃博霉素B的基本工艺参数,具体考察了接种量、搅拌转速、通气量、添加消泡剂及补糖等5个工艺参数对埃博霉素B发酵产量的影响。最后确定发酵罐基本发酵条件为接种量9%,搅拌转速180 r/min,空气流量3.5 L/min,消泡剂种类选择Antifoam B聚醚类消泡剂,补糖控制在发酵液糖浓度为0.2 g/L,在此条件下埃博霉素B的产量可达25.6 mg/L。

Fermentation Conditions of Epothilone B in Pilot Fermenter

In order to improve the production of epothilone B, the effects of basic technological parameters of fermentation of Sorangium cellulosum SoF5-76 were studied in a 5 L pilot fermenter, concretely inoculation volume, stir rmp, aeration rate, addition of defoaming agent, and sugar addition were investigated. Finally it was confirmed that basic fermentation condition of the fermenter were as follows: inoculation volume 9%, stir rmp at 180, aeration rate at 3.5 L/min, selected B polyether defoamer as defoaming agent, and sugar concentration in fermentation broth was controlled at 0.2 g/L. Under these optimum fermentation conditions, the yield of epothilone B was as high as 25.6 mg/L.