2株海洋来源产浅蓝霉素A的异壁放线菌的分离和鉴定

利用抗菌及卤虫致死活性模型,从中国南海海底沉积物来源的微生物中筛选到2株放线菌SCSIO WJ01和SCSIO ZJ63,其发酵产物具有较强活性,经16S rRNA基因序列分析这2株放线菌均为异壁放线菌Actinoalloteichus sp.。HPLC-DAD分析显示2株放线菌能产生同一个主要的次级代谢产物,通过正相硅胶柱色谱、反相中压柱色谱、半制备高效液相色谱等手段,从SCSIO WJ01的发酵产物中分离获得了该化合物,运用ESI-MS、1H及13C NMR波谱分析鉴定为浅蓝霉素A(Caerulomycin A)。此外,还从SCSIO WJ01的发酵产物中分离鉴定了浅蓝霉素D。

Isolation and Identification of Two Marine Originated Actinoalloteichus sp. Producing Caerulomycin A

Two marine originated actinobacteria strains SCSIO WJ01 and SCSIO ZJ63 isolated and screened from sediments collected in the bottom of South China Sea, using antibiotic and lethal activities model against brine shrimp (Artemia salina), their fermentation broths possessed fairly strong activity. These two strains were identified as Actinoalloteichus sp. through 16S rRNA gene sequence analysis. HPLC-DAD analysis disclosed that strains SCSIO WJ01 and SCSIO ZJ63 could produce the same major secondary metabolite. These fermentation products were isolated from the fermentation extract of strain SCSIO WJ01 by means of ortho phase silica gel column chromatography and counter phase mid pressure column chromatography, and semi preparative HPLC etc and identified to be caerulomycin A using ESI-MS,¹H and ¹³C NMR spectroscopic analyses. In addition, caerulomycin D was also isolated and identified from fermentation product of strain SCSIO WJ01.