Cellular localization and developmental changes of deoxyribonucleoside-activated nucleotidase in the rat testis |
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| Authors: | T B Haugen P Fritzson V Hansson |
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| Institution: | 1. CNR-IAS, via de Marini 6, Genova 16149, Italy;2. National Biodiversity Future Center (NBFC) S.c.a.r.l., Piazza Marina 61 (c/o palazzo Steri), Palermo, Italy;3. Universidad Nacional de Educación a Distancia (UNED), Madrid, Spain;4. Costa Edutainment SpA - Acquario di Genova, Area Porto Antico, Ponte Spinola, Genoa 16128, Italy;1. School of Basic Medical Science, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan 750004, China;2. Institute of Reproductive Medicine, School of Medicine, Nantong University, Nantong 226001, China;3. State Key Laboratory of Reproductive Medicine and Offspring Health, Center for Reproduction and Genetics, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School of Nanjing Medical University, Suzhou 215002, China |
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| Abstract: | Specific deoxyribonucleoside-activated nucleotidase (DAN) activity showed a rapid decline during the exponential increase in testis weight between 25 and 35 days of age. Specific DAN activity in Sertoli cells was dependent on the amount of cytosol in the enzyme assay. At optimal cytosol concentration the measured value was 50 units/mg of protein. Specific DAN activity in peritubular cells, primary spermatocytes and round spermatids was 13, 3.7 and 3.1 units/mg, respectively, and was independent of the cytosol concentration in the assay. |
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