Search for genetic variation in the blood of Norwegian dairy goats reveals a new polymorphism at the HbβA locus |
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Authors: | M. BRAEND E. M. TUCKER S. W. CLARKE |
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Affiliation: | Department of Internal Medicine I, Norwegian College of Veterinary Medicine, Oslo, Norway;AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK |
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Abstract: | A total of 150 blood samples tested for serum albumin and transferrin and for red cell carbonic anhydrase, phosphoglucomutase, phosphogluconate dehydrogenase, phosphohexose isomerase, nucleoside phosphorylase, acid phosphatase, 'X'-protein and potassium concentration only showed variation at the 'X' protein and nucleoside phosphorylase loci. Isoelectric focusing over pH range 6-8 showed 145 samples to be of haemoglobin type A and 5 type AD. The haemoglobin A type was resolved into further types by separation over pH 6.9-7.5 in Immobiline polyacrylamide gels. A 2- or 4-band pattern was present in 136 of the samples; a genetic hypothesis based on four or more different haemoglobin A variants is proposed. 14 samples had a 3-, 5- or 6-band pattern. It is assumed that these are heterozygous for a variant of the II alpha gene. |
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Keywords: | goat haemoglobin βA globin polymorphism |
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