Inhibition by mepacrine and amylase secretion from intact and permeabilized rat pancreatic acini. |
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| Authors: | K Grosfils F Gomez J P Dehaye |
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| Institution: | Department of General and Human Biochemistry, Free University of Brussels, Belgium. |
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| Abstract: | In intact rat pancreatic acini, the phospholipase A2 inhibitor mepacrine did not affect basal amylase release but dose-dependently inhibited the carbachol (IC50 65 microM) and CCK-8 (IC50 210 microM)-stimulated amylase release. In permeabilized acini, mepacrine shifted the dose-response curve for calcium to the right by a factor 2 and inhibited the release of amylase stimulated by GTPrS. From these results we conclude that carbachol, CCK-8 and GTPrS probably activate a phospholipase A2 closely coupled to exocytosis. |
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