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ATPase activity of sunflower root membranes as affected by flooding
Authors:Habanta Mukherjee  David Drakeford  David M. Reid
Affiliation:Plant Physiology Research Group, Dept of Biology, Univ. of Calgary, Calgary, Alberta, Canada T2N 1N4.
Abstract:Flooding of excised roots or roots of intact aeroponically grown sunflower ( Helianthus annuus L. cv. Russian) caused a reduction in the activity of ATPases of microsomal membrane vesicles within 1 day. With increasing flooding of up to 3 days, the enzyme activity in the roots declined. During the same period, enzyme activity gradually increased in the non-flooded aeroponically grown roots. Continuous aeration during flooding either maintained the activity of the enzyme to the level of the controls or enhanced it. The ATPase did not require MgSO4 and had a much higher activity at pH 6.7 than at pH 8.5. The activity of the ATPases was markedly inhibited by sodium orthovanadate. There was 8 to 44% stimulation of the activity of the ATPases due to KCl in the absence as well as presence of MgSO4. These data indicate that a substantial proportion of the ATPases, and thus the interface vesicles, could be of plasma membrane origin.
Keywords:Aerobic    anaerobic    Helianthus annuus    waterlogging
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