Physical mapping of the lysozyme gene family in cattle |
| |
| Authors: | Daniel S Gallagher Jr David W Threadgill Anne M Ryan James E Womack David M Irwin |
| |
| Institution: | (1) Department of Veterinary Pathobiology, Texas A&M University, 77843 College Station, Texas, USA;(2) Center for Animal Genetics, Institute of Biosciences and Technology, Texas A&M University, 77843 College Station, Texas, USA;(3) Department of Genetics, Case Western Reserve University, School of Medicine, 44106 Cleveland, Ohio, USA;(4) Department of Clinical Biochemistry, Banting and Best Diabetes Centre, University of Toronton, 100 College Street, M5G 1L5 Toronto, Ontario, Canada |
| |
| Abstract: | Amplification of an ancestral lysozyme gene in artiodactyls is associated with the evolution of foregut fermetation in the ruminant lineage and has resulted in about ten lysozyme genes in true ruminants. Hybridization of a cow stomach lysozyme 2 cDNA clone to restricted DNAs of a panel of cowxhamster hybrid cell lines revealed that all but one of the multiple bovine-specific bands segregate concordantly with the marker for bovine syntenic group U3 Chromosome (Chr) 5]. The anomalous band was subsequently mapped to bovine syntenic group U22 (Chr 7) with a second panel of hybrids representing all 31 bovine syntenic groups. By two-dimensional pulsed-field gel electrophoresis the lysozyme genes on cattle Chr 5 were shown to be clustered on a 2- to 3-Mb DNA fragment, while the lactalbumin gene and pseudogenes that are paralogous and syntenic with the lysozymes were outside the lysozyme gene cluster. Chromosomal fluorescence in situ hybridization of a cocktail of lysozyme genomic clones localized the lysozyme gene cluster to cattle Chr 5 band 23, corroborating the somatic cell assignment. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
